Restriction Enzymes
New England Biolabs, Inc. NheI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence G^CTAGC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. AflIII – 250 units
A restriction endonuclease that recognizes the sequence A^CRYG_T.
New England Biolabs, Inc. KasI – 250 units
A restriction endonuclease that recognizes the sequence G^GCGC_C.
New England Biolabs, Inc. BciVI – 200 units
A restriction endonuclease that recognizes the sequence GTATCCNNNNN_N^.
New England Biolabs, Inc. TaqI-v2 – 4000 units
A restriction endonuclease that recognizes the sequence T^CG_A.
New England Biolabs, Inc. BstAPI – 200 units
A restriction endonuclease that recognizes the sequence GCAN_NNN^NTGC.
New England Biolabs, Inc. XRN-1 – 100 units
XRN-1 is highly processive 53 exoribonuclease, requiring 5 monophosphate. It also acts on 5' monophosphate ssDNA with greatly reduced efficiency.
New England Biolabs, Inc. Nt.BbvCI – 1000 units
A restriction endonuclease that recognizes the sequence CC^TCAGC.
New England Biolabs, Inc. XbaI – 3000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. BstXI – 1000 units
A restriction endonuclease that recognizes the sequence CCAN_NNNN^NTGG.
New England Biolabs, Inc. AscI – 2500 units
A restriction endonuclease that recognizes the sequence GG^CGCG_CC.
New England Biolabs, Inc. Bsu36I – 1000 units
A restriction endonuclease that recognizes the sequence CC^TNA_GG.